Project Title
Optimization of CRISPR/cas9 System in Agrobacterium tumefaciens Targeting proC2
Document Type
Event
Start Date
10-5-2019 3:30 PM
End Date
10-5-2019 6:30 PM
Description
Our research has focused on adapting the CRISPR/cas9 system to create a proC2 mutant strain of Agrobacterium tumefaciens C58. We aim to find the optimal length of homology to the organism's genome in comparison to an inserted fragment of DNA to drive homology directed repair. Through this we are able to create a proC2 gene knockout to be used in further studies of gene duplication.
Discipline
Biology
Research Mentor(s)
Dr. Derek Wood
Copyright Status
http://rightsstatements.org/vocab/InC/1.0/
Additional Rights Information
Copyright held by author(s).
Optimization of CRISPR/cas9 System in Agrobacterium tumefaciens Targeting proC2
Our research has focused on adapting the CRISPR/cas9 system to create a proC2 mutant strain of Agrobacterium tumefaciens C58. We aim to find the optimal length of homology to the organism's genome in comparison to an inserted fragment of DNA to drive homology directed repair. Through this we are able to create a proC2 gene knockout to be used in further studies of gene duplication.