Project Title
Constructing bioengineered proC2 mutants through in vivo E. coli cloning
Document Type
Event
Start Date
10-5-2019 3:30 PM
End Date
10-5-2019 6:30 PM
Description
Constructing bioengineered Agrobacterium tumefaciens C58 proC2 mutants through in vivo E. coli cloning to observe variations in proline biosynthesis. We are observing the efficiency of our homology arms compared to CRISPR/Cas9 in creating mutants. Mutants can later be used to test different environmental stressors. Differences in proline production can help us better understand the relationship between ProC1 and ProC2 genes and answer the big question of why multiple proC genes are found in wild-type agrobacterium.
Discipline
Biology
Research Mentor(s)
Dr. Derek Wood
Copyright Status
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Additional Rights Information
Copyright held by author(s).
Constructing bioengineered proC2 mutants through in vivo E. coli cloning
Constructing bioengineered Agrobacterium tumefaciens C58 proC2 mutants through in vivo E. coli cloning to observe variations in proline biosynthesis. We are observing the efficiency of our homology arms compared to CRISPR/Cas9 in creating mutants. Mutants can later be used to test different environmental stressors. Differences in proline production can help us better understand the relationship between ProC1 and ProC2 genes and answer the big question of why multiple proC genes are found in wild-type agrobacterium.
Rights Statement
