Project Title

Quantification of cellular free proline using the colorimetric ninhydrin assay and the Standard Addition Method

Document Type

Event

Start Date

10-5-2019 3:30 PM

End Date

10-5-2019 6:30 PM

Description

Using the Standard Addition Method, a simple analytical chemistry technique, our research shows that the classical colorimetric ninhydrin assay (commonly used for determining amino acid concentrations within cells) can be used to determine intracellular proline in a dependable range to do a qualitative and quantitative comparison of two different ProC genes within a complex cellular background.

Discipline

Biology

Research Mentor(s)

Derek Wood

Copyright Status

http://rightsstatements.org/vocab/InC/1.0/

Additional Rights Information

Copyright held by author(s).

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May 10th, 3:30 PM May 10th, 6:30 PM

Quantification of cellular free proline using the colorimetric ninhydrin assay and the Standard Addition Method

Using the Standard Addition Method, a simple analytical chemistry technique, our research shows that the classical colorimetric ninhydrin assay (commonly used for determining amino acid concentrations within cells) can be used to determine intracellular proline in a dependable range to do a qualitative and quantitative comparison of two different ProC genes within a complex cellular background.

Rights Statement

In Copyright