Digital Commons @ SPU - Erickson Undergraduate Research Conference: Quantification of cellular free proline using the colorimetric ninhydrin assay and the Standard Addition Method
 

Project Title

Quantification of cellular free proline using the colorimetric ninhydrin assay and the Standard Addition Method

Document Type

Event

Start Date

10-5-2019 3:30 PM

End Date

10-5-2019 6:30 PM

Description

Using the Standard Addition Method, a simple analytical chemistry technique, our research shows that the classical colorimetric ninhydrin assay (commonly used for determining amino acid concentrations within cells) can be used to determine intracellular proline in a dependable range to do a qualitative and quantitative comparison of two different ProC genes within a complex cellular background.

Discipline

Biology

Research Mentor(s)

Derek Wood

Copyright Status

http://rightsstatements.org/vocab/InC/1.0/

Additional Rights Information

Copyright held by author(s).

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May 10th, 3:30 PM May 10th, 6:30 PM

Quantification of cellular free proline using the colorimetric ninhydrin assay and the Standard Addition Method

Using the Standard Addition Method, a simple analytical chemistry technique, our research shows that the classical colorimetric ninhydrin assay (commonly used for determining amino acid concentrations within cells) can be used to determine intracellular proline in a dependable range to do a qualitative and quantitative comparison of two different ProC genes within a complex cellular background.

Rights Statement

In Copyright