Project Title
Assessing genetic diversity in an island deer population
Document Type
Event
Start Date
10-5-2019 3:30 PM
End Date
10-5-2019 6:30 PM
Description
Island populations are vulnerable to reduced fitness due to small population sizes. This study analyzes two indirect markers of fitness, male survivorship and mitochondrial genetic diversity in Black-tailed deer (Odocoileus hemionus columbianus) by extracting DNA from bone. Sex-specific and mitochondrial primers were used to amplify sequences of interest, then PCR products were analyzed using gel electrophoresis. We will present preliminary data for sex identification of deer, and discuss challenges to and optimization strategies for amplifying products from bone DNA."
Discipline
Biology
Research Mentor(s)
Jennifer Tenlen
Copyright Status
http://rightsstatements.org/vocab/InC/1.0/
Additional Rights Information
Copyright held by author(s).
Assessing genetic diversity in an island deer population
Island populations are vulnerable to reduced fitness due to small population sizes. This study analyzes two indirect markers of fitness, male survivorship and mitochondrial genetic diversity in Black-tailed deer (Odocoileus hemionus columbianus) by extracting DNA from bone. Sex-specific and mitochondrial primers were used to amplify sequences of interest, then PCR products were analyzed using gel electrophoresis. We will present preliminary data for sex identification of deer, and discuss challenges to and optimization strategies for amplifying products from bone DNA."