Project Title

Development of Agrobacterium tumefaciens C58 mutants via the CRISPR/Cas9 system to test proC1 (ATU-2209) function

Document Type

Event

Start Date

10-5-2019 3:30 PM

End Date

10-5-2019 6:30 PM

Description

Without the production of proline from its surrounding environment, A. tumefaciens has two processes allowing for the biosynthetic creation of proline. Our research looks at the duplicity of these processes by creating a bacterial mutant using CRISPR as genetic editing tool. We hope to successfully create a mutant that has knocked out one of the two proC genes within A. tumefaciens to be able to further investigate why this species has retained two replicate genes.

Discipline

Biology

Research Mentor(s)

Dr. Derek Wood

Copyright Status

http://rightsstatements.org/vocab/InC/1.0/

Additional Rights Information

Copyright held by author(s).

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May 10th, 3:30 PM May 10th, 6:30 PM

Development of Agrobacterium tumefaciens C58 mutants via the CRISPR/Cas9 system to test proC1 (ATU-2209) function

Without the production of proline from its surrounding environment, A. tumefaciens has two processes allowing for the biosynthetic creation of proline. Our research looks at the duplicity of these processes by creating a bacterial mutant using CRISPR as genetic editing tool. We hope to successfully create a mutant that has knocked out one of the two proC genes within A. tumefaciens to be able to further investigate why this species has retained two replicate genes.

Rights Statement

In Copyright