Project Title
Development of Agrobacterium tumefaciens C58 mutants via the CRISPR/Cas9 system to test proC1 (ATU-2209) function
Document Type
Event
Start Date
10-5-2019 3:30 PM
End Date
10-5-2019 6:30 PM
Description
Without the production of proline from its surrounding environment, A. tumefaciens has two processes allowing for the biosynthetic creation of proline. Our research looks at the duplicity of these processes by creating a bacterial mutant using CRISPR as genetic editing tool. We hope to successfully create a mutant that has knocked out one of the two proC genes within A. tumefaciens to be able to further investigate why this species has retained two replicate genes.
Discipline
Biology
Research Mentor(s)
Dr. Derek Wood
Copyright Status
http://rightsstatements.org/vocab/InC/1.0/
Additional Rights Information
Copyright held by author(s).
Development of Agrobacterium tumefaciens C58 mutants via the CRISPR/Cas9 system to test proC1 (ATU-2209) function
Without the production of proline from its surrounding environment, A. tumefaciens has two processes allowing for the biosynthetic creation of proline. Our research looks at the duplicity of these processes by creating a bacterial mutant using CRISPR as genetic editing tool. We hope to successfully create a mutant that has knocked out one of the two proC genes within A. tumefaciens to be able to further investigate why this species has retained two replicate genes.